Faculty
Geng, Weitao
Li, Chao
Li, Hongbo
Li, Hongjuan
Li, Ping
Li, Zhenjing
Liu, Huanhuan
Lu, Laifeng
Meng, Demei
Qiao, Liping
Sui, Wenjie
Wang, Dongjie
Wang, Shuai
Wang, Tianxin
Wu, Shufen
Yang, Chen
Yao, Yunping
Zhang, Qin
Zhang, Xiaowei
Zhang, Xiaoxu
Zhang, Yan
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Meng, Demei

  

Demei Meng

Ph.D, Lecture

address:

Yifulou Building, College of Food Engineering & Biotechnology, Tianjin University of Science and Technology, TEDA, Tianjin, 300457, China

email:

mengdm@tust.edu.cn

phone:

+86 022-60912418

Biography

Dr. Meng received Bachelor Degree from Qingdao Agricultural University in 2009 and then completed MD-PhD study and received Doctor Degree from China Agricultural University in 2014. On the completion of her PhD, Dr. Meng is employed by Tianjin University of Science & Technology as a lecture.

Research Interests

Dr. Meng has been conducting researches that aim to maintain postharvest quality of fruits and vegetables, especially edible mushrooms. As the poor preservation technology has become an important bottleneck restricting the development of edible fungi industry, research on developing safe and effective preservation methods of edible fungi have important theoretical and practical significance. Methyl jasmonate (MeJA), as a kind of plant signal molecule, has significant positive effects on the quality retention of postharvest fruits and vegetables. Hence, one of her research program is focused on the roles and mechanisms of MeJA/polyamines in the postharvest quality regulation of Agaricus bisporus mushroom. To make clear of the mechanisms, iTRAQ-based quantitative proteomics were applied, combined with techniques including western blotting, real-time quantitative PCR, structural simulation, site-directed mutagenesis, and so on.

She also conduct research on recombinant preparation, purification and charaterization of various antimicrobial peptides (AMPs). Preparative isolation of antimicrobial peptides from natural sources is not an economically efficient way since a large quantity of antimicrobial peptides is potentially required for applications. By taking advantage of eukaryotic protein expression system, Pichia pastori as a suitable cell factory system of high efficiency was used to produce functional recombinant AMPs. To better understand if the yeast-produced AMP could be applied in food preservation, medicine, cosmetic, veterinary or aquaculture, the production yield, antimicrobial activity, stability, haemolytic acticity, cytotoxic activity, and mechanisms of action were investigated. Many techniques including SEM, TEM, fluorescence microscope, mass spectrum, ITC, CD, and etc. were used.  

Recent Publications

[1] Meng Demei, Zhang Yaxuan, Yang Rui, et al. Arginase participates in the methyl jasmonate-regulated quality maintenance of postharvest Agaricus bisporus fruit bodies, Postharvest Biology and Technology, 2017, 132: 7-14.

[2] Meng Demei, Zhao Jingfang, Ling Xiao, Dai Hongxia, Guo Yajun, Xiao-Fang Gao, Dong Bin, Zhang Ziqi, Meng Xin, Fan Zhenchuan. Recombinant expression, purification and antimicrobial activity of a novel antimicrobial peptide PaDef in Pichia pastoris, Protein Expression and Purification, 2017, 130: 90-99.

[3] Shujuan Wu, Ying Nie, Jinhong Zhao, Bei Fan, Xiaofei Huang, Xianxian Li, Jiping Sheng, Demei Meng, Yang Ding, Xuanming Tang. The synergistic effects of low-concentration acidic electrolyzed water and ultrasound on the storage quality of fresh-sliced button mushrooms, Food & Bioprocess Technology, 2017 (2).

[4] Meng Demei, Dai Hongxia, Gao Xiaofang, et al. Expression, Purification and Initial Characterization of a novel recombinant antimicrobial peptide Mytichitin-A in Pichia pastoris, Protein Expression and Purification, 2016, 127: 35-43.

[5] Yang Ding, Zhen Zhu, Jinhong Zhao, Ying Nie,Yu Zhang, Jiping Sheng, Demei Meng, Hongmei Mao, Xuanming Tang. Effects of postharvest brassinolide treatment on the metabolism of white button mushroom (Agaricus bisporus) in relation to development of browning during storage, Food & Bioprocess Technology, 2016, 9 (8) :1327-1334.

[6] Meng Demei, Song Tianzi, Shen Lin, et al. Postharvest application of methyl jasmonate for improving quality retention of Agaricus bisporus fruit bodies, Journal of Agricultural and Food Chemistry, 2012, 60 (23): 6056-6062

[7] Meng Demei, Shen Lin, Yang Rui, et al. Identification and active site analysis of the 1-aminocyclopropane-1-carboxylic acid oxidase catalysing the synthesis of ethylene in Agaricus bisporus, Biochimica et Biophysica Acta-General Subjects, 2014, 1840 (1): 120-128.

[8] Meng Demei, Shen Lin, Zhang Xinhua, et al. Isolation of high quality and yield of RNA from Agaricus bisporus with a simple, inexpensive and reliable method, Biotechnology Letters, 2012, 34 (7): 1315-1320.

[9] Yang Rui, Meng Demei (contribute equally as the first author), Hu Xiaosong, et al. Saccharification of pumpkin residues by coculturing of Trichoderma reesei RUT-C30 and Phanerochaete chrysosporium Burdsall with delayed inoculation timing, Journal of Agricultural and Food Chemistry, 2013, 61 (38): 9192-9199.

[10] Zhang Xinhua, Shen Lin, Li Fujun , Meng Demei, Sheng Jiping , Hot air treatment-induced arginine catabolism is associated with elevated polyamines and proline levels and alleviates chilling injury in postharvest tomato fruit, Journal of the Science of Food and Agriculture, 2013, 93 (13)3245-3251.

[11] Zhang Xinhua, Shen Lin, Li Fujun, Meng Demei, Sheng Jiping, Arginase induction by heat treatment contributes to amelioration of chilling injury and activation of antioxidant enzymes in tomato fruit, Postharvest Biology and Technology, 2013, 791-8.

[12] Zhang Xinhua, Shen Lin, Li Fujun , Meng Demei, Sheng Jiping, Amelioration of chilling stress by arginine in tomato fruit: Changes in endogenous arginine catabolism, Postharvest Biology and Technology, 2013, 76106-111.

Teaching

 Food quality evaluation for undergraduate students