Yifulou Building, College of Food Engineering & Biotechnology, Tianjin University of Science and Technology, TEDA, Tianjin, 300457, China
Dr. Mingfei Pan is an associate professor of Food Engineering and Biotechnology College, Tianjin University of Science and Technology. Dr. Pan completed Bachelor, Master and Doctor Degrees from Tianjin University of Science and Technology at 2006, 2009 and 2012. and obtained a postdoctoral position in Tianjin University of Science and Technology in 2013-2015. Since 2015, Dr. Pan is employed by Tianjin University of Science & Technology as a lecturer and promoted to an associate professorn in 2017.
(1) Molecularly imprinted materials are a new type of functional materials with high selectivity, wide environment and controllable synthesis. In the field of food safety inspection, especially in the purification of complex matrix, the enrichment and separation of trace harmful substances and the rapid detection of biomimetic sensing, it has a wide application prospect. However, due to the limitation of synthesis and modification methods, the selectivity of existing MIM materials is not obvious enough, and the matching performance with existing analytical instruments is poor, which can not meet the application needs. The mechanism of the imprinting and recognition of MIM is not clear. Based on this, Dr. Pan first proposed the "Stereospecific imprinting" concept of potential induced "directional imprinting". The specific selection and recognition ability of molecularly imprinted materials on sensing surface has been effectively improved by potential induction and molecular structure regulation (4-6 times of the original method). The synthetic process has high controllability and good stability. It is concluded that the potential interval and intensity are the important conclusions that determine the key factors of the selection and recognition performance of Electrodeposited MIM material, and reveal the mechanism of molecular imprinting and recognition to a certain extent.
(2) The stability and effective conversion of sensor recognition signals is a key problem in designing a new high performance biomimetic and biosensor for food safety detection. Signal stability and conversion effectiveness determine the performance of the sensor directly, and then affect the accuracy and sensitivity of the sensor in food safety detection. Carbon based or gold based nanomaterials and transition metal complexes have large specific surface area, excellent biocompatibility, electrical conductivity and catalytic ability, which can increase the electron transfer efficiency of transducer and enhance signal stability and conversion efficiency. Based on this, the bionic (molecular imprinting) transducer and immune sensing substrate orderly doped with carbon nanotubes, gold nanoparticles, Salen-Co (III) composite materials, electron transfer barriers to overcome sensor recognition sites or components in the complex system in disorder caused by the discovery of nanoparticles contributes to the orderly arrangement of electron transfer, sensor the signal was enhanced (increased 3-4 times), the detection limit can be reduced to nearly 100 times the harmful substances in food. The results explain the essence of nanomaterials improving the signal transduction ability of complex sensing system, and provide powerful support for the design of new highly stable and highly sensitive food safety bionic and biosensing detection system.
(3) The sensing recognition binding site can be identified and combined directly with the target, and its number directly affects the sensitivity of food safety sensing detection. The dendrimer of organic synthesis belongs to a class of "protein like" materials. Because of its biocompatibility due to its surface charge, the stability of the immunosensing substrate with antibody as recognition element is improved, and the problem of abscission and deactivation of biological macromolecules is avoided. A large number of active groups on the surface can also increase the number of recognition sites of organic synthetic materials, and play an important role in the design and construction of highly sensitive bionic and immunosensor. Based on this, Dr. PAN creatively introduced polyamines - amines as functional monomers into the molecularly imprinted polymerization system. As an immunosensing substrate, it is used to immobilize and maintain the antibody recognition molecules, increase the adsorption amount, and reach the ng level for the detection limit of the harmful substances. The research results put forward a new theory for the design and construction of high performance and high sensitivity food safety bionics and immunosensors.
(1) Mingfei Pan, Ying Gu, Miyao Zhang, Junping Wang, Yaguang Yun, Shuo Wang. Reproducible molecularly imprinted QCM sensor for accurate, stable, and sensitive detection of enrofloxacin residue in animal-derived foods. Food Analytical Methods, 2017, (4): 1-9.
(2) Mingfei Pan, Shijie Li, Junping Wang, Wei Sheng and Shuo Wang. Development and validation of a reproducible and label-free surface plasmon resonance immunosensor for enrofloxacin detection in animal-derived foods. Sensors,2017,17(9), 1984.
(3) Mingfei Pan, Ying Gu, Yaguang Yun, Min Li, Xincui Jin and Shuo Wang. Nanomaterials for electrochemical immunosensing. Sensors,2017,17(5), 1041.
(4)Mingfei Pan, Xiaojun Wang, Junping Wang, Yang Lu, Kun Qian, Shuo Wang. Stable and sensitive detection of sulfonamide residues in animal-derived Foods using a reproducible surface plasmon resonance immunosensor. Food Analytical Methods, 2017, 10(6): 2027-2035.
(5) Mingfei Pan, Guozhen Fang, Lingjie Kong, Yukun Yang, Jie Dai, Shuo Wang*. Molecularly imprinted biomimetic QCM sensor involving a poly(amidoamine) dendrimer as a functional monomer for the highly selective and sensitive determination of methimazole. Sensors and Actuators B: Chemical, 2015, 207, 588-595.
(6)Mingfei Pan, Lingjie Kong, Bing Liu, Kun Qian, Guozhen Fang, Shuo Wang*. Production of multi-walled carbon nanotube / poly(aminoamide) dendrimer hybrid and its application to piezoelectric immunosensing for metolcarb. Sensors and Actuators B: Chemical, 2013, 188, 949-956.